Rhodamine-Phalloidin 羅丹明標(biāo)記鬼筆環(huán)肽（橙紅）- 50T

貨號(hào)：JXF40201-50T

規(guī)格：50T

價(jià)格：800元

Product Description

Phalloidin is a bicyclic peptide that belongs to a family of toxins isolated from the deadly Amanita phalloides mushroom. 

Fluorescent phalloidins bind F-actin with nanomolar affinity and are water soluble, thus providing convenient probes 

for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections, cell cultures, or cell-free experiments. 

Phalloidin contains an unusual thioether bridge between cysteine and tryptophan residues that forms an inner ring structure. 

At elevated pH, this thioether is cleaved and the toxin loses its affinity for actin. Fluorescently labeled phalloidins stain F-actin 

at nanomolar concentrations. Labeled phalloidins have similar affinity for both large and small filaments, binding in a  

stoichiometric ratio of about one phalloidin molecule per actin subunit in muscle and non-muscle cells from various species 

of plants and animals. Different from antibodies, the binding affinity of phalloidin does not change significantly with actin 

 among different species. Non-specific staining is negligible, and the contrast between stained and unstained areas is 

extremely large. Phalloidin shifts the monomer/polymer equilibrium toward the polymer, lowering the critical concentration 

for polymerization up to 30-fold. Phallotoxins also stabilize F-actin, inhibiting depolymerization by cytochalasin, 

potassium iodide and elevated temperatures. Because the phalloidin conjugates are small, with an approximate diameter 

of 12-15? and molecular weight of <2000 Daltons, a variety of actin-binding proteins including myosin, tropomyosin and 

troponin can still bind to actin after  treatment with phalloidin. Even more significantly, phalloidin-labeled actin filaments 

remain functional; labeled glycerinated muscle fibers still contract, and labeled actin filaments still move on solid-phase 

myosin substrates. Fluorescent phalloidin can also be used to quantify the amount of F-actin in cells.