Source

Clostridium histolyticum

Specific Activity

>125 u/mg solid

Specificity

Clostridial collagenase I or clostridiopeptidase A degrades the helical

regions in native collagen preferentially at the Y-Gly bond in the

sequence -Pro-Y-Gly-Pro- where Y is most frequently a neutral amino

 acid. This bond in synthetic peptide substrates may also be split.

Storage and Handling

Store at +4 °C. Store Desiccated.

Unit Definition

One unit liberates one μmole of L-leucine equivalents from collagen in

5 hours at 37 °C, pH 7.5