線性PEI轉染試劑(25K)
英文名 Polyethylenimine Linear(PEI) MW25000
貨號 JX23966
保存條件 室溫
概述
線性PEI轉染試劑是一種陽離子聚合物,分子量25000,它能與核酸形成復合物,并使該復合物進入哺乳動物細胞。線性PEI轉染試劑廣泛適用于常見細胞系,如 HEK-293、HEK293T、 Hep G2、Hela、CHO-K1、COS-1、COS-7、NIH/3T3 和 Sf9 等。該試劑即使在有血清存在的情況下,它仍然能高效的將核酸導入細胞。
我公司提供的線性PEI轉染試劑具有如下優點:優越的轉染效率 , 重組蛋白的高表達水平,與含血清的培養基相兼容, 低細胞毒性,易于操作。
Transfection Reagent Preparation and Storage Recommendations
These guidelines are our recommendations based off research, testing, and customer feedback. There are alternative methods available, but we suggest following these recommendations for easy adoption and consistently high performance. These methods are adapted from Cold Spring Harbor Protocols, an excellent secondary resource for transient transfection literature.
Reagent Preparation (1 mg/mL)
Materials:
6.Sterile HDPE or polypropylene storage vials for reagent aliquots.
Equipment
8.Aliquot as desired and store at -20℃.
Reagent Storage
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